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Impacto del petróleo crudo sobre grupos funcionales de bacterias cultivables y colonización de microorganismos simbióticos en la rizosfera de Clitoria-Brachiaria crecidas en mesocosmos. Montecillo what is set class 11, Texcoco, Estado de México, México. This research evaluated the changes on populations of culturable Best pizza brooklyn new york photos free bacteria NFFB and P-solubilizing bacteria PSBas well as on the root nodulation by native rhizobia, the root colonization and spore number of arbuscular mycorrhizal fungi AMFin the rhizosphere of Clitoria ternatea and Brachiaria brizantha grown in mesocosms contaminated with crude oil 0,and mg kg -1for days.
The highest number of root nodules in C. At 60 days, AMF colonization in both plant species, and the number of spores significantly decreased as the crude oil concentration increased; however, at days, the highest number of AMF spores was recorded at treatments with and mg kg The dry weight of both plant species significantly decreased as crude oil concentrations increased. Examples of mutualistic relationship between bacteria and other organisms C.
Keywords: Arbuscular mycorrhizal fungi; crude oil; N-fixing free bacteria; P-solubilizing bacteria; root nodulation; soil contamination. El examples of mutualistic relationship between bacteria and other organisms seco vegetal disminuyó significativamente al aumentar las concentraciones de petróleo crudo. Palabras clave: Bacterias fijadoras de N de vida libre; bacterias solubilizadoras de P; contaminación de suelo; hongos micorrízicos arbusculares; nodulación en raíz.
Plant rhizosphere harbors several microbial groups whose physiological activity significantly influences soil fertility, quality, and health properties De Ridder-Duine et al. This rhizosphere interaction not only benefits the microbial communities but also influences positively both plant growth and adaptation Walker et al. Soil microflora is mainly represented by bacteria and fungi Dajoz et al. Bacteria may release organic compounds, some of them may establish symbiosis with plants, and others may inhibit the proliferation of plant pathogens due to secretion of antibiotic compounds Barea, ; Ferrera-Cerrato and Alarcón, ; Mitter et al.
Microorganisms play a significant role in nutrient cycling in soil such as biological examples of mutualistic relationship between bacteria and other organisms nitrogen fixation or solubilization of inorganic phosphates, whose deficiency typically impairs plant growth and what are the application of linear equations in real life Barea, Also, arbuscular mycorrhizal fungi AMF are obligated biotrophic symbionts that colonize cortical cells of roots of most of the extant terrestrial plants, and enhance plant nutrition and growth, as well as plant adaptation against stressful soil conditions, water deficiency, contamination, or pathogens Linderman, ; Jeffries et al.
These fungi have important effects during the phytoremediation of soils contaminated with petroleum hydrocarbons Cabello, by enhancing plant adaptation, growth, nutrition or by stimulating the proliferation of petroleum-degrading microorganisms in the rhizosphere Joner and Leyval, ; Alarcón et al. The later benefits examples of mutualistic relationship between bacteria and other organisms the crucial role of rhizosphere microorganisms by improving physical and chemical properties in the surrounding edaphic environment Zhang et al.
Soil pollution by accidental oil spills is an environmental issue that has received special attention worldwide. These contaminants modify soil properties by forming a layer covering the surface and the pore space, thus affecting oxygen diffusion Franco et al. Likewise, hydrocarbons decrease water retention due to their hydrophobic properties, and significantly increase the amount of carbon, induce acidification processes, and decrease the cation exchange capacity Li et al.
When hydrocarbons accumulate in the rhizosphere the most affected physiological process in the plant is photosynthesis so that the chlorophyll content decreases in leaves Adenipekun et al. These contaminants exert pressures on the floristic composition, favoring the selection of well-adapted plant species. Part of this adaptation consists in their association with soil microorganisms as a mechanism to withstand the adverse conditions caused by contaminants Franco et al.
Besides causing toxic effects to many microorganisms, some oil fractions are utilized as a source or carbon and energy for satisfying microbial growth Franco et al. These are evident on culture-dependent microorganisms, by which is possible the characterization and the selection of microorganisms with potential use for bioremediation of soils contaminated with several compounds Alkorta et al. The responses of AMF to petroleum hydrocarbons are related to reducing root colonization.
However, AMF may stimulate plant survival growing at contaminated conditions, and allow the proliferation of microorganisms able to degrade organic compounds Binet et al. The ability of plants to grow at contaminated media varies from one species to another, and this variation is the key for the remediation of soils contaminated with petroleum hydrocarbons Akutam et al. Clitoria ternatea L. Rich are plant species of tropical regions, easy to establish, resistant to drought, and tolerant to organic contaminants Sangabriel et al.
However, the rhizosphere examples of mutualistic relationship between bacteria and other organisms populations in both plant species under contaminated soils are not well studied. Thus, the present study evaluated the effects of crude oil contaminated soil on the culture-dependent population of rhizosphere microorganisms, whose physiological activity is related to the incorporation of atmospheric nitrogen, the solubilization of inorganic phosphates in the soil, and the promotion of can intercept be negative in regression of two plant species established in mesocosms under greenhouse conditions.
Eight kilograms of dry sieved soil 2 mm mesh were placed in each of the 15 plastic containers 36 x 30 x 14 cm used as mesocosms. The soil was artificially contaminated with crude oil at the following concentrations:,and mg kg -1respectively. A treatment without oil pollution was included as a control. One week after contamination, 15 seeds of Clitoria ternatea L. Fabaceae and 15 of Brachiaria brizantha Hochst.
Examples of mutualistic relationship between bacteria and other organisms were planted in combination in each mesocosm evenly distributed in the substrate at one-centimeter depth. Throughout the experiment days under greenhouse conditions, the mesocosms were irrigated with tap water as needed. The temperature and relative humidity maximum and minimum prevailing during this research were The population of functional groups of culturable bacteria and root colonization of symbiotic microorganisms.
Soil sampling was collected at 60 and days. For each mesocosm, a composite soil sample was prepared from five sampled points g rhizosphere soil each. Thus, three composite samples per treatment were obtained. From these composite samples, 10 g examples of mutualistic relationship between bacteria and other organisms soil were used for determining culturable microorganisms according to serial dilutions and agar plate counting technique Lorch et al.
For C. The mycorrhizal colonization in both C. Once the roots were dyed and mounted on slides, the frequency of AMF structures hyphae, vesicles, and arbuscules in each root fragment was estimated using a clear field optical microscope Reichert, Microstar Model at 40 X magnification, and to calculate the percentage of colonization.
The undamaged spore counting was done under a stereomicroscope Reichert, StereoStar Zoomand results were expressed as a number of examples of mutualistic relationship between bacteria and other organisms per g dry soil. Assessment of phototoxicity of oil in Clitoria ternatea and Brachiaria brizantha. At the end of the experiment daysthe toxicity of the crude oil to plants was evaluated by quantifying the dry biomass and the total chlorophyll content in leaves of both species.
The total dry weight of C. The total chlorophyll content was determined by the method described by Dere et al. One leaf of C. Subsequently, from the obtained solution, absorbance readings were taken, and nm, in a spectrophotometer Hewlett Packard, model The experiment consisted of five treatments with three replicates each, distributed in a completely randomized experimental design. The percentages of mycorrhizal colonization were transformed to arcsine units, while the values from meaning of cholai tamil word in english quantification of bacterial CFU were transformed to log units for subsequent statistical analysis.
At days, the NFFLB population was significantly higher in treatments with and mg kg -1 than the control Fig. Figure 1 Populations of a N-fixing free-living bacteria N2-FFLB and b P-solubilizing bacteria in the rhizosphere of Clitoria ternatea and Brachiaria brizantha grown in mesocosms contaminated with four concentrations of crude oil, at an initial time 24 h after contaminationwhat is meaning of greenhouse effect in punjabi and days.
The number of nodules in C. At 60 days, control plants had in average 25 nodules, whereas at contaminated treatments, plants had in average four nodules Fig. At days, control plants showed 27 nodules, while concentrations of and mg kg -1 resulted in a low number of nodules 7 Fig. In contrast, treatments with and mg kg -1resulted in the lowest number of nodules Fig.
Figure 2 Number of nodules formed by native rhizobia in roots of Clitoria ternatea aand number of spores b of arbuscular mycorrhizal fungi AMF in g of dry soil collected from the rhizosphere of Clitoria ternatea and Brachiaria brizantha grown mesocosms contaminated with four concentrations of crude oil, after 60 and days. Mycorrhizal colonization at 60 days in B. At days, B. At days, C. Figure 3 Arbuscular mycorrhizal colonization in roots of Clitoria ternatea and Brachiaria brizantha grown in mesocosms contaminated with four concentrations of crude oil, after 60 a and c and b and d days.
After 60 days, control treatment had the highest number of AMF spores spores in g dry soilbut in contaminated treatments, the number of spores ranged from 55 to 28, without presenting statistical differences among treatments Fig. In contrast, the number of spores at days was significantly higher at the concentration of mg kg -1 spores than that from mg kg -1 45 spores Fig. Phytotoxic effects of crude oil on Clitoria ternatea and Brachiaria brizantha.
The total dry weight of B. At days, the highest dry weight was obtained in control plants and the lowest in plants grown under mg kg -1 Fig. Figure 4 Shoot dry mass of Brachiaria brizantha and Clitoria ternatea grew in mesocosms contaminated with four concentrations of crude oil, after 60 a and c and b and d days. For B. However, after days, the total chlorophyll content significantly decreased in all treatments, although the significant highest content was obtained in plants exposed to mg kg -1 Fig.
In contrast, at days, plants grown in treatments with and mg kg -1 had significantly higher chlorophyll content than the remaining treatments Fig. Figure 5 Total chlorophyll content in leaves of How to fix internet connection not secure brizantha and Clitoria ternatea grew in mesocosms contaminated the soil with four concentrations of crude oil, after 60 a and c and b and d days.
Increasing concentrations of crude oil caused significant reduction of bacterial populations at initial sampling time, thus, proving the negative effects of this contaminant, which also acts as a selective toxic agent. Chikere et al. Also, microorganisms compete for available nutrients and energy sources; thereby the microbial population is restricted Miranda-Martínez et al.
The growth of bacterial populations may be due to the selective effects of crude oil on soil microorganisms, favoring those with the ability to degrade or utilize petroleum hydrocarbons as a source of carbon and energy Delille et al. For example, Proteobacteria and Actinobacteria are dominant bacterial groups in contaminated soils and able to metabolize hydrocarbons Yang et al. Delille et al. Furthermore, these processes are regulated by both physicochemical properties of hydrocarbons and environmental conditions Stroud et al.
Although no analyses of hydrocarbon degradation were performed, the described microbial processes explain in part the significant recovery of bacterial populations after 60 days. Undoubtedly, tap water irrigation may contribute with the addition of some microorganisms to mesocosms, but petroleum hydrocarbons acted as a selective agent from the beginning of experimentation, so at the end time daysthis contaminant acted on the nutrition courses in uae examples of mutualistic relationship between bacteria and other organisms stabilization of well-adapted NFFLB and PSB populations, and on the increased colonization of AMF and nodule-forming rhizobacteria.
This phenomenon was described by Liste and Felgentreu, with legumes and grasses in fields exposed to petroleum hydrocarbons. Crude oil significantly affected the formation of nodules by native rhizobia in roots of C. Few studies have described the negative effects of petroleum hydrocarbons on rhizobial nodulation; overall nodulation decreases in those legumes exposed to petroleum hydrocarbons, either at controlled or natural conditions Lindström et al.
At days, nodulation in Clitoria plants showed certain recovery at mg kg In this regard, petroleum hydrocarbons may promote the N-fixation by rhizobial nodules in legumes grown in contaminated boreal soils Yan et al. In this regard, the functionality of nodules in terms examples of mutualistic relationship between bacteria and other organisms leghemoglobin content pink coloration or nitrogenase activity was considered in the present study since the aim of this research was focused on the expression of nodules in roots due to the recovery of native rhizobia in the soil.
Either AMF colonization or number of spores decreased significantly as crude oil concentrations increased, which concurs with negative effects of petroleum hydrocarbons on AMF Cabello, ; Gaspar et al. However, our results denote that AMF sporulation showed a recovery at days Figure 2 band AMF colonization also increased in roots of Brachiaria when grown in treatments with to mg examples of mutualistic relationship between bacteria and other organisms -1at days Figure 3 b.
The sporulation represents an AMF strategy to ensure their progeny under environmentally stressful conditions; moreover, AMF colonization may increase plant resistance to abiotic stresses Harrier and Watson, Phytotoxicity of crude oil resulted in decreased dry biomass of Brachiaria, and more dramatically in Clitoria, which was more susceptible to contamination, excepting examples of mutualistic relationship between bacteria and other organisms 60 days at mg kg -1 when its biomass increased, probably due to the nutrient availability provided for the proliferation of PSB, for instance.
Excepting this, the results concur to those effects described for several plant species including non-legume or legume species under contaminated soils at greenhouse conditions Adenipekun et al.
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