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Spatial phylogenetics in Hechtioideae Bromeliaceae reveals recent diversification and dispersal. La filogenética espacial de Hechtioideae Bromeliaceae revela diversificación y dispersión reciente. Ivón M. Juan P. Katya J. In recent years, evolutionary relationships within this lineage have been studied; however, the biogeography of these plants have not yet been explored from a phylogenetic framework. The integration of geographic and phylogenetic information in the evolutionary study of organisms has facilitated the identification of patterns, as well as the exploration of new hypotheses that allow for the understanding the processes that have influenced the evolutionary history of lineages.
What aso in german translation the biogeographic history of this lineage? How Hechtioideae has diversified over time? The Neotropical region has the highest species richness of Hechtioideae and the Mexican Transition Zone is the area with the greatest phylogenetic diversity. This lineage presented its highest diversification rate during the late Miocene and Pleistocene 6.
The ancestral area of the group corresponds to the Neotropical region and the Mexican Transition Zone. In addition, Hechtioideae spread across its current ranges through multiple dispersal events associated with climatic and geological events during the last 10 Ma. Hechtioideae is a group of recent origin whose evolutionary history has been strongly influenced by geological and climatic events over the past 10 Ma, such as the glacial and interglacial periods of the Pleistocene and the great tectonic and volcanic activity that led to the formation pros and cons of phylogenetic species concept the Trans-Mexican Volcanic Belt.
Keywords: Ancestral area reconstruction; biogeography; conservation; distribution; phylogenetic diversity. Este linaje presentó su mayor tasa de diversificación durante el Mioceno tardío y el Pleistoceno 6. Hechtioideae is one of eight subfamilies of Bromeliaceae Givnish et al. Romero Ramírez-Morillo et al. These plants have terrestrial and lithophytic habits, usually growing on volcanic or karst rocks. They all grow as a rosette and have distinctive growth patterns inflorescence origin, production of new shoots, etc.
Figure 1 Morphological characteristics of Hechtioideae and examples of habitats where it is present. A Bakerantha purpusii growing on cliff. B Hechtia schottii. D Mesoamerantha dichroantha growing on rocks. Image credits: A, D by K. Romero-Soler; Pros and cons of phylogenetic species concept, C by I. According to Givnish et al. Its current range is restricted to this region, stretching across a territorial extension ranging from the deserts of Chihuahua and Sonora south to northern Nicaragua.
Most species have restricted distribution ranges and high endemism rates Ramírez-Morillo et al. In recent years, efforts have been made to determine the evolutionary history of this group of plants by analyzing their phylogenetic relationships and the description and delimitation of their species García-Ruiz et al. These advances have made possible to identify the monophyly of Hechtioideae and the presence of three well-supported clades corresponding to three genera; Bakerantha with five species, Hechtia with ca.
However, there are still evolutionary and biogeographic aspects that have not been fully studied, for example, the diversification process in the lineage and the identification of areas of higher or lower diversity. The answers to these questions may improve our understanding of the events that have shaped the evolutionary history of the group and identify and propose areas for the conservation of these threatened plants.
The analysis of biogeographic patterns at different scales has enabled us to test hypotheses to explore ecological and evolutionary processes influencing the conformation of the biota Steinbauer et al. For a long time, studies aimed to identify these patterns by analyzing taxonomic diversity, with the species level as the basic and equivalent unit for measuring biological diversity only, and disregarding the evolutionary, functional, and morphological characteristics that make lineages different Hillebrand et al.
Thus, phylogenetic diversity PD arises as a statistic that allows the analysis of biological diversity considering the evolutionary relationships and cumulative changes in each species over time Faith This concept has given rise to multiple indices and methodologies where the phylogenetic distance measurement is used as a tool to identify the historical and environmental processes that give rise to biotic assemblages Webb et al. Although the incorporation of phylogenetic information into the study of spatial diversity is relatively recent, several hypotheses have already been proposed about biogeographic patterns and biotic assemblage mechanisms that consider evolutionary relationships FaithFaith et al.
Similarly, conservation biology has used this new phylogenetic approach to maximize what does having a clean room mean efforts focused on safeguarding not only as many species as possible, but also the greatest extent of evolutionary history Purvis et al. However, multiple species have been described since then, and a fairly robust phylogenetic hypothesis is now available to implement metrics that assess the spatial pattern of lineages by incorporating the evolutionary relationships within the group.
The present research assesses the spatial phylogenetics of the subfamily Hechtioideae across pros and cons of phylogenetic species concept distributional range. Our goals were to analyze the phylogenetic assemblage processes in the Hechtioideae throughout biogeographic areas and recognize key areas for its conservation. Geographic distribution data and areas for assessment. Georeferenced records of the Hechtioideae were obtained from a comprehensive literature revision that included protologues of all the species in the group, general and specific taxonomic treatments for particular states of Mexico and natural areas Pulido-Esparza et al.
Records with no exact coordinates, but with detailed pros and cons of phylogenetic species concept of the collection site were georeferenced with the program Google Earth Pro v. A total of 94 species were considered; of these, five have not yet been described but were included because there is sufficient molecular and morphological information available to consider them in the phylogenetic analysis. The geographic range of the Hechtioideae was divided into three areas, namely the Nearctic, Neotropical, and Mexican Transition Zone biogeographic regions sensu Morrone et al.
Estimation of divergence times. A phylogenetic hypothesis was constructed for 75 species out of the 94 species recognized in this study of Hechtioideae. We included sequences for 50 species of Hechtioideae from chloroplast pros and cons of phylogenetic species concept intergenic spacer rpl32 - trnL and the ycf1 gene and nuclear PRK gene DNA regions previously generated by Ramírez-Morillo et al.
We added sequences for other 25 species using the same primers and DNA extraction and amplification protocols as Ramírez-Morillo et al. The sequences generated were reviewed and assembled in Geneious v. The nucleotide substitution model pros and cons of phylogenetic species concept was the one suggested what is the purpose of phylogenetic tree jModelTest v.
The analysis was performed using a relaxed clock model with an uncorrelated lognormal distribution, pros and cons of phylogenetic species concept the Meaning of partnership class 12 speciation model. Because of the lack of fossils records for the family Bromeliaceae, we used previously estimated dating times to calibrate the phylogeny, using the crown group age of Hechtioideae, at Two runs of 50, generations were performed, sampling every 5, generations.
Pros and cons of phylogenetic species concept results were reviewed with Tracer v. Additionally, pros and cons of phylogenetic species concept species with no information for any molecular sequence were included manually to counter the fact that the lack of lineages underestimated the true phylogenetic pool for each set of species as highlighted by Sandel To this end, we identified those clades including species with geographic affinity in relation to missing taxa, as the Hechtioideae shows strong patterns of endemism and geographic restriction Ramírez-Morillo et al.
The species were linked to the basal node of each respective clade. This addition produced some polytomies, all of them in the terminal parts of the tree. This methodology helps to compensate for the loss of phylogenetic information derived from an incomplete phylogenetic hypothesis of the group. Diversity metrics and phylogenetic structure. Phylogenetic structure was calculated using the standardized effect size phylogenetic diversity Proches et al.
These indices consider the average phylogenetic distance pros and cons of phylogenetic species concept pairs of taxa; therefore, they do not apply for areas with less than two species. Ancestral area reconstruction. The reconstruction of ancestral areas was carried out with RASP v. The Nearctic, Neotropical, and Mexican Transitional Zone biogeographic regions were selected as the reconstruction states. The potential ancestral ranges were estimated for each node of the phylogenetic tree.
The Hechtioideae chronogram was used as a consensus tree. The number of maximum areas remained at three. Ten MCMC chains were run simultaneously for 5, generations and the reconstructed state was sampled every 1, generations. Geographic distribution. A total of 2, records of Hechtioideae were gathered, of which 8. These records identified the distribution range of Hechtioideae, which is restricted to the Megamexico III biogeographic region Figure 2.
Hechtia is the genus with the widest distribution within the Hechtioideae, spreading from the south of the USA throughout the Mexican territory south to at least Honduras, whereas the genera Bakerantha and Mesoamerantha have more restricted distributions, covering certain areas in central Mexico and Central America, respectively.
Figure 2 Territorial extension of Megamexico III demarcated by red lines and the different provinces that make up the Nearctic and Neotropical biogeographic regions, as well as the Pros and cons of phylogenetic species concept Transition Zone within the area. The dots on the map correspond to records considered to determine the distribution range of each species. The chronogram generated for Hechtioideae includes 94 of the 95 species in the group Figure 3.
The only species that was not included in the phylogenetic analysis was Hechtia reticulata L. Figure 3 Chronogram for Hechtioideae derived from the Bayesian analysis of chloroplast ycf1 and rpl32 - trnL and nuclear PRK regions; the species included manually are marked in red. According to the chronogram recovered from the phylogenetic analyses, the ancestral lineage of Hechtioideae underwent a diversification process during the Mid-Miocene, with a crown age of One corresponds pros and cons of phylogenetic species concept the ancestral lineage of Hechtiawhich has a crown age of 6.
Currently, this lineage includes 87 species clustered in various clades. The other large lineage corresponds to the ancestor of the Bakerantha - Mesoamerantha clade, which has a crown age of 9. The Mesoamerantha lineage has a crown age of 6. Finally, the Bakerantha lineage has a crown age of 5. For SES. At the biogeographic province level, the California province C does not harbor any species of Hechtioideae, while the Sonoras SYucatan Yand El Mosquito M provinces each have a single species.
A NRI values by biogeographic regions. B NRI values by biogeographic provinces. C NTI values by biogeographic regions. D NTI values by biogeographic provinces. The values in the boxplot correspond to the results of each province belonging to the different regions. Positive values suggest phylogenetic clustering; negative values suggest phylogenetic overdispersion. The results of the Bayesian Binary Analysis MCMC BBM suggest that the biogeographical history of Hechtioideae has been complex, since what is a non identity function reconstruction of the ancestral area for many of the nodes including pros and cons of phylogenetic species concept ancestor of all Hechtioideae does not correspond to a single region but to combined areas multi-areas.
It reached its current range what are the 4 types of bases in dna 60 dispersal events, 13 vicariance events, and 2 extinction events Figure 5A. The reconstruction analysis reveals that the largest diversification in the group occurred around 3.
A Reconstruction of ancestral areas by node; colors at the nodes of each pie chart indicate the percentage of reconstruction for each area.
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