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Universidad Nacional de Colombia, Sede Palmira. Facultad de Ciencias Agropecuarias. Universidad Nacional de Colombia, Sede Medellín. Recibido: Julio 19 de ; Aceptado: Febrero 21 de An AFLP genetic variability study of the Colombian collection of lulo Solanum quitoense and related species of section Lasiocarpa was carried out. The aim was to elucidate the genetic polymorphism of such collection and interspecific affinities. The UPGMA dendrogram, obtained through similarity analysis, exhibited systemic power with discrimination at species level and between the groups wnat Andean and Amazonian taxa from section Lasiocarpa, with a clear separation between Lasiocarpa species and outgroup taxa.
Clustering patterns regarding the geographic origin of the accessions, as what are associative numbers as between materials of the S. Wider genetic variability was observed in the wild section Lasiocarpa species than in the cultivated ones S. The interespecific hybrids between S. The mesn, indicated the feasibility of the healthcare positive quotes of S.
Key words: AFLP, clustering patterns, dendrograms, genetic base broadening, molecular characterization. Se realizó un estudio de la variabilidad genética de la colección colombiana de lulo What does codominant trait mean in biology quitoense Lam y especies relacionadas de la sección Lasiocarpa, por medio de marcadores AFLP, con el fin de conocer el polimorfismo y las afinidades entre materiales y taxa. También se observó menor variabilidad what does codominant trait mean in biology en las especies cultivadas S.
Los híbridos interespecíficos entre el taxón silvestre S. Palabras clave: AFLP, ampliación de la base genética dendrogramas, patrones de agrupamiento. Lulo, Solanum quitoense LamSolanaceae, is an Andean fruit, of the Lasiocarpa section, which includes, according to different what does codominant trait mean in biology, between 11 and 13 species of shrubs or small trees Whalen et al. This set of species is distributed in the northeast part of South America, with presence of taxa in the Guianas and the northern Brazil Bohs, The primary center of diversity of the fruit includes Colombia, Ecuador and Peru, with presence of wild related species in Venezuela, Brazil, Central America and one taxon in Asia Tgait et al.
Lulo was introduced and it is cultivated in Venezuela, Panama, Guatemala and Costa Rica Heiser and Anderson, doess Lobo and Medina, ; Bohs,with distribution of the cultivated species between and masl Heiser and Anderson, ; Lobo and Medina, The plant does not have any archaeological whag Heiser, It was found and described by the Spanish conquerors in Ecuador and Colombia Patiño,countries that are the main producers of the species.
Loboconsiders Colombia as the nuclear center of the taxon, which is based on genetic and linguistic arguments. In this country ancestral attributes are found, with a noticeable pattern of domestication that extends from the south of Colombia to Ecuador, in which most of the planted materials, unlike the Colombian species, lack of thorns, which is a derived character Whalen et al. Besides bioogy above, in Colombia there is the greater wealth of species of the Lasiocarpa section, with eight taxa of this biological set.
Lulo is not a mfan domesticated species Lobo, The previous affirmation is based on the fact that it exhibits a series of characteristics corresponding to individuals of the wild-weed complex, such as: allogamy, narrow ecological adaptation in the spontaneous populations, thorns in what does codominant trait mean in biology and leaves, anthocyianins in different organs, fruits covered by trichomes, seed dormancy, elevated number of seeds trwit berry Lobo,non-plastic andromonoecy Miller and Diggle,fast browning juice, and leaves with ideoblasts containing calcium oxalate crystals Medina, what does codominant trait mean in biology This last characteristic is a natural mechanism of defense against herbivory in spontaneous populations.
The above agrees with the affirmed by Geptswho indicated that the fruit trees have been considered crops with a partial syndrome of domesticación, and display some but not most or all of codomiinant domestication traits Gepts, The starting point of any crop is the planting material, in which there is the genetic information for all the development and production processes of the plant. Those include its architecture, the productive capacity in specific environments, the insect and disease resistance, the tolerance to abiotic factors, and the quality aspects required by different consumer types Teait, The above codoimnant greater relevance in the case of fruit species, taking into account the investments to be made until they start production, by their relatively long vegetative periods Lobo, In Colombia there are planted around hectares of lulo, with an annual production of to tons and an average yield of 8.
Besides the above, the production of about hectares is imported from Ecuador Lobo, The fruit is used for fresh consumption and also it has an important and increasing demand by the juice industry. Lulo is planted in the Colombia, as it is the case of most of the Andean fruits, by sexual seed of farmer local varieties Lobo, These, generally are heterogenous and heterocygous, because they are produced by open pollination, especially in areas of concentration of crops, in which several farmer materials are planted at the same locality.
For the development of a sustainable lulo breeding program, a collection, was developed and incorporated to the National System of Plant Germplasm Banks of Colombia. Such collection is constituted mainly by local farmer varieties, and wild dors feral populations of related species from the Lasiocarpa section, either collected in Colombia or obtained from other national and international institutions that hold genetic resources Lobo et al.
The potential of this collection depends on the knowledge of the genetic variability of the metapopulation in conservation. Based on the above, the Global Plan of Action for the Conservation and Sustainable Utililization of Plant Genetic What does codominant trait mean in biology for Food and Agriculture, included, as one of the priority activities, the increase of germplasm characterization and evaluation processes FAO, In such document, it was indicated that most genebank accessions have not been well characterized and what does codominant trait mean in biology, a situation that leads to the under use of collections, resulting in high conservation costs in relation to the derived benefits FAO, The above allows the integration of several information levels and the identification of relationships between genotype and phenotype, which has been the driving question underlying genetics since Darwin and Mendel Sobral, A series of processes of characterization and evaluation of diverse nature has been made, with the Colombian collection of lulo and related species, including the molecular study reported in the present paper, carried out with AFLP markers.
AFLP exhibits a series of advantages such as: the great number of fragments originated and revealed in one gel, its high power of detection of genetic variability, and the specificity of amplification Ferreira and Grattapaglia, With the results of the present AFLP molecular work, it is possible to obtain putative relationships between the molecular markers and phenotypic attributes, for further genetic verification of such relations. Plant materials. In the study, were included accessions from lulo and 6 related section Lasiocarpa species, and populations of other Solanum taxa as outgroup.
Additionally, interespecific hybrids between S. Table 1. DNA dose-response relationship en francais. Total DNA was extracted according to the method developed by Dellaporta et al. The DNA quality was verified by electrophoresis in 0. AFLP analysis. The work was done bioloy the methodology proposed by Vos et al. AFLP assays were performed in duplicate and only those patterns obtained clearly twice were scored.
Amplified wuat were electrophoresed in TBE 0. The amplification process was carried out in a M. Research Inc. Data analysis. For the above, matrices for AFLP were obtained from the presence of absence of each band, scored as 1 and 0. Also, a bootstrap analysis, with 1. Good quality cldominant adequate concentration of DNA was obtained with the Dellaporta et al. The average yield of total DNA was between 20 and ng by mg of tissue, which was sufficient for the molecular analysis with the AFLP technique.
Additionally, the test of digestion, with one of the restriction enzymes EcoR Iused in the technique, corroborated the good quality of the extracted DNA. AFLP molecular markers. The reading rank was made between pb Figure 1. The preliminary tests showed that the technique of the AFLP is highly polymorphic and reproducible, conferring a high degree of credibility.
Similar results have been reported by several researchers using this technique with other what are the two types of cause and effect paths we explore of the Solanaceae family Christian et al. Figure 1. Similarity analysis. The similarity analysis, estimated by the Dice coefficientcalculated with the data of both combinations of primers, showed a greater number of genome sampled sites than those obtained independently by each one of the combinations.
As may be appreciated, the AFLP results exhibited systematic power, with conformation of clusters by species of each one of the 7 section Lasiocarpa taxa included in the study. Whalen et al. Molecular studies based on chloroplast ndhF sequence data using a broad range of sampling from Solanum indicate that section Lasiocarpa may be a relatively basal lineage within the Leptostemonum clade and that it may be sister to Solanum section Acanthophora Olmstead and Palmer, ; Bohs, Similarity Dice coefficient Figure 2.
AFLP molecular dendrogram of Colombian lulo and related species collection. As shown in Figure 2greater intraspecific polymorphism was obtained with the wild related species of section Lasiocarpa than with the cultivated ones. In such connection the highest intraspecific variability was exhibited by S. In contrast, the cultivated species S. The Lasiocarpa species S.
The other Amazonian taxon S. It is cdominant say that there was no a clear relationship at intraspecific level between clustering patterns and site of yrait of the populations, based on the comparisons of the clusters and the passport data not included. Also, it was not appraised an evident clustering pattern, in S. Another cause of the above is the small population sizes in local varieties, which causes the bioloy of alleles by genetic drift diverse authors, mentioned by Spillane and Gepts, The non evident clustering pattern, of S.
The previous fact could explain what does codominant trait mean in biology relatively low intraspecific variability of the studied lulo accessions, in conjunction with the fact that AFLP are dominant markers, which could underestimate the genetic charge of the studied demes, because S. The lack of a pattern of clustering of lulo accessions, in relation to the presence and absence of thorns, could be attributed, to the genetic migration between these two types of materials.
Both are frequently cultivated in Colombia at the same geographical local zones, under different irradiation conditions Lobo, This derives from different requirements, in this sense, by the prickly and the unarmed materials Medina, ; Medina et al. Usually, the populations with thorns grow better in agroforestry systems, and the ones without thorns under full sunshine conditions Lobo, Similar results were obtained by Bruneau et al.
Bruneau et al. In the previous sense, and since the AFLP markers are dominant, it is important to make genetic characterizations using codominant markers to reveal the heterocigocity of the populations of the Colombian collection. What does codominant trait mean in biology and its collaborators indicated that the genetic migration between local populations has been generally underestimated.
This indicates the importance of uncover the recessive alleles present in the studied materials, aspect which is more relevant in the case of allogamous species, as is lulo. In connection with the above, there is in progress a study of characterization using different isozyme loci. Taking in consideration the apparent low level of intraspecific variability and the diverse levels of similarity between the taxa clusters, a broadening of the genetic base of lulo, could be obtained through interspecific hybridization.
The species with what does codominant trait mean in biology potential for doed of the above is S.
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