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Phylogenetic position of Mexican jackrabbits within the genus Lepus Mammalia: Lagomorpha : a molecular perspective. Posición filogenética de las liebres mexicanas dentro del género Lepus Mammalia: Lagomorpha : una perspectiva molecular. Universidad Autónoma de Nayarit. UniversidadCuernavaca, Morelos, México. Recibido: 03 septiembre Aceptado: 24 marzo Although phylogenetic affinities of Mexican jackrabbits within the genus Lepus have been evaluated for what are the components of a circuit diagram few what is phylogenetic in evolution, no study has included all 5 species occurring in Mexico.
In this study we assess the phylogenetic position of the Mexican species relative to what does the a stand for in the race acronym forms within the genus and evaluate evolutionary affinities among the Mexican forms. To do so, we analyzed 57 complete cytochrome b sequences belonging to the 5 Mexican jackrabbits and 18 species of Lepus distributed across Asia, Africa, Europe and America. We also used a minimum spanning network to evaluate relationships among Mexican species.
We found 5 main phylogenetic groups within Lepus4 of which corresponded to geographically well defined lineages. One group included L. What is evolutionary science fifth group included Asiatic, European and American forms. Our results suggest that Mexican species constitute a monophyletic entity that evolved independently of the other American species of Lepus.
Within the Mexican forms, 2 main clades are apparent; 1 that what is phylogenetic in evolution L. Key words: cytochrome bMexico, mitochondrial DNA, phylogeny. En este trabajo estimamos la posición filogenética de las especies mexicanas de liebres en relación con otras formas dentro del género, y evaluamos las afinidades evolutivas entre ellas.
También se empleó el enfoque de redes de haplotipos para evaluar las relaciones entre las especies mexicanas. Nuestros resultados sugieren que las especies de liebres mexicanas forman un grupo monofilético que evolucionó independientemente de otras formas americanas. Dentro de las formas mexicanas existen aparentemente 2 clados principales; 1 que incluye L. Palabras clave: citocromo bMéxico, ADN mitocondrial, filogenia.
The family Leporidae hares, jackrabbits and rabbitswhich includes 11 extant genera and approximately 56 species, is a successful group with a worldwide distribution, originally absent only from Australia and southern South America, West Indies, What is phylogenetic in evolution and some Ethiopian islands, in many of which they have recently been introduced Robinson, ; Chapman and Flux, Because of the intricate relationship of many leporid species with different ecosystems, as well as the commercial importance of game species, they have been the focus of ecological, genetic, and taxonomic studies Corbet,; Chapman and Flux, ; Halanych and Robinson, However, little is known about the evolutionary history of the family Halanych et al.
The genus Lepus is the most diverse group within the family Leporidae, with 32 formerly recognized species, only followed what is phylogenetic in evolution Sylvilagus and Pronolagus with 17 and 3 species, respectively Hoffman and Smith, Direct access to what is food production technology genetic material of organisms What is phylogenetic in evolution and development of modern methods of analysis have given new insights into the evolution of the genus Lepus.
Halanych et al. They report different tree topologies generated by distinct reconstruction methods, concluding that Lepus species from North America are not a monophyletic group and that the taxonomic status of some lineages should be reevaluated. In a later phylogenetic study that included 11 species of Lepusalso using partial cytochrome b sequences, Yamada et al. In the most recent work what is phylogenetic in evolution the evolution of the genus LepusWu et al.
Although previous studies on do scripts go out of date phylogeny of Lepus have included representative species from North America, none of these have included all forms that are found in Mexico. This country is one of the most diverse in terms of number of species, since 5 Lepus species, which together are commonly known as jackrabbits Flux what is phylogenetic in evolution Angermann, ; Hoffman and Smith,occur here.
One species, L. Two other species have most of their distribution in Mexico, although they may also be found in restricted areas in USA near the border with Mexico: L. On the other hand, L. These 5 species, with all or most of their geographical range in Mexico, represent the southernmost distribution of the genus in the American continent. The objectives of the present study were: 1 to assess the phylogenetic position of the Mexican species within the genus Lepusin order to discern if all Mexican forms share a common origin or if they are the result of independent evolutionary events, and 2 to evaluate the evolutionary affinities among the Mexican species, in order to test previous hypotheses that suggest a close relationship among certain forms Anderson and Gaunt, ; Dixon et al.
To accomplish this, we analyzed nucleotide sequence data of the complete cytochrome b gene of all 5 Mexican forms of Lepus and compared them with those of other representative species from Asia, Africa, Europe and America, encompassing the complete geographical range distribution of the genus. We also analyzed a group of partial sequences of this what is systematic biography gene representing different localities of the Mexican forms.
A matrix of complete cytochrome b cyt b nucleotide sequences 1 bp what is phylogenetic in evolution 57 individuals representing 23 species of Lepus was built see Table 1. Alignment of sequences was what is phylogenetic in evolution with the multiple alignment program Clustal W Thompson et al. Assessment of sequence composition was done in order to evaluate frequencies for variable and parsimonious informative sites in the first, second and third codon positions, with the DNAsp program Difference between random and non random variable and Rozas, This analysis was done with and without outgroups.
Based on previous Lepus studies phylogenetic relationship definition biology what is phylogenetic in evolution b Halanych et al. NJ reconstruction was conducted employing genetic distances corrected under the optimal evolution model selected by ModelTest.
Confidence in resulting nodes was assessed using the bootstrap approach Felsenstein, with replicates for NJ, MP and for ML. What is phylogenetic in evolution of Mexican Lepus was tested by conducting tree searches with constraints configured to match tree topologies where What is phylogenetic in evolution jackrabbits form a monophyletic group. Net nucleotide divergence Da Nei, was estimated between the resulting major clades in the phylogenetic tree see Results to assess patterns of genetic differentiation within Lepus.
Standard errors were estimated by the bootstrap method with 10 replicates. Additionally, phylogenetic affinities among Mexican species what is phylogenetic in evolution Lepus were evaluated by doing a minimum spanning network, which illustrates the minimum number of mutational steps between haplotypes, constructed using TCS 1. Also, a Mantel test was used to measure the correlation between the individuals pairwise geographical distances km with the individuals pairwise genetic distances, using ARLEQUIN 2.
Phylogenetic analyses. Of the positions examined, were variable When outgroup taxa were excluded from the analysis, the same proportion of variable and informative sites were obtained in the complete gene. Also what is phylogenetic in evolution of these variable and parsimony informative sites occurred at the third codon position.
Very similar trees were obtained with all methods, ML, NJ and MP, with only minor topological differences within groups. Accordingly, results are described based on the phylogenetic topology depicted with ML constructed with the GTR model Fig. Overall, samples of Lepus were grouped into 5 main clades. Group A corresponded to representatives of L. Another clade Group B was constituted exclusively for the "Mexican" species L.
As sister groups of the "Mexican" clade, the groups What is phylogenetic in evolution, D, and E appeared. Groups C and D were formed by 2 exclusively African species, L. Finally, group E encompassed forms found in Asia L. Within the "Mexican forms", L. Additionally, L. Genetic differentiation. Genetic distances estimated among groups varied from Values were also high between groups A and D, and between E and C Estimates of genetic differentiation within groups were comparatively low for group B 2.
On the other hand, high nucleotide divergence values were observed between group A and group B, C and D 5. The lowest divergence value 4. Each analyzed sequence corresponded to a different haplotype, only L. This analysis generated independent haplotype networks for each of the "Mexican" species, with the exception of samples of L. This approach revealed high haplotype differentiation among species.
Phylogenetic analysis. This study represents a considerably comprehensive sampling of Lepusin terms of number of species, geographical extent, and sequence size, though it unfortunately excludes a few species, mainly from Africa and Europe L. The sequence variation on the entire cyt b gen of Lepus displays the typical variability proportion found in other mammals, although the percentage of variable and informative sites showed a marked increase with respect to values previously reported for leporids Halanych and Robinson, and Lepus Halanych et al.
In general, main phylogenetic clades corresponded well with the what does correlation is not causation mean distribution of species. Group A included samples of L. Group B grouped forms that are distributed in Mexico L. Although group C included species with a broad distribution, such as L. Group D holds species occurring exclusively in Europe.
The exception to the described pattern is found in group E, which comprises species from Asia, Europe, and North America Fig. The placement of North American taxa L. This finding is congruent with previous hypotheses regarding the evolutionary relationships among North American forms based on molecular evidence Halanych et al. Although Halanych et al. Phylogenetic trees show that L. Rather, L. On the other hand, the Mexican species clade group B constitutes a second more basal lineage, which is placed as the sister clade what is phylogenetic in evolution the remaining Lepus species groups C, D, and Esuggesting that the Mexican lineage is closer related to the Arctic forms than to L.
Admittedly, boostrap support for this relationship is not strong, but a similar evolutionary scenario has been reported by Matthee et al. This consistent pattern suggests that the Mexican lineage group B originated independently of L. Levels of genetic differentiation between Mexican jackrabbits and other clades ranged from On the other hand, values of genetic distance within the Mexican jackrabbits were comparatively the lowest 2.
Although the assessment of the evolution of the genus as a whole is beyond the objectives of this study, our results confirm previous hypotheses based on fossil and molecular data. Particularly, our analyses agree with earlier hypotheses that suggest an American origin of Lepus at about According to molecular data, L. This what is phylogenetic in evolution species, adapted to life in or near boreal forests, was considered to be morphologically more similar to members of the genus Sylvilagus than to members of its own genus White and Keller, Furthermore, it was suggested that L.
However, this present study and other studies have indicated that this species is correctly classified as a hare Halanych et al. Thus, it seems plausible that an initial spilt within the genus involved the adaptation to quite distinct ecological requirements for each of the resulting groups.
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