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E-mail: cmgalosi fcv. Journal of veterinary medicine. A total of serum samples were selected from December to March DOI :. Español es Select your language. Last news of research. Citation Type.

Genomic study of Argentinean Equid herpesvirus 1 strains. Nadia A. Fuentealba 1, 3Guillermo H. Sguazza 1Matías L. Eöry 1, 2, 3Alejandro R. Valera 1Marcelo R. Pecoraro 1Cecilia M. E-mail: cmgalosi fcv. Equid herpesvirus 1 EHV-1 infection has a signifcant economic impact on equine production, causing abortion, respiratory disease, neonatal death and neurological disorders.

The identifcation of specifc EHV-1 genes related to virulence and pathogenicity has been the aim of several research groups. The is tough love good in a relationship of the present study was to analyze different genomic regions of Argentinean EHV-1 strains and to determine their possible relationship with virulence or clinical signs.

Twenty-fve EHV-1 Argentinean isolates recovered from different clinical cases between and and two reference strains were amplifed horzes sequenced. The sequence alignments were carried out using Clustal X version 1. Minor changes were observed. No changes that could be involved in the different virulence in the mouse model of three EHV-1 Argentinean strains were found. No genetic horsse were observed. The genomic regions analyzed are unsuitable for differentiation between abortigenic strains and those isolated from neonatal deaths.

Key words: Equid herpesvirus 1; Genomic analysis; Virulence. Estudio genómico what is ehv 4 in horses cepas argentinas de Herpesvirus equino 1. La infección por Herpesvirus equino 1 EHV-1 tiene un signifcativo impacto económico en la producción equina mundial al causar abortos, enfermedad respiratoria, muertes perinatales y desórdenes neurológicos. La identifcación de genes específcos relacionados con la virulencia y patogenicidad de este virus ha sido el propósito de varios grupos de investigación.

En este trabajo se analizaron diferentes regiones genómicas de what is ehv 4 in horses argentinas de EHV-1 para determinar la posible relación entre la estructura genómica y la virulencia o los signos clínicos producidos. Veinticinco cepas aisladas de diferentes casos clínicos observados entre los años y y dos cepas de referencia fueron amplifcadas y secuenciadas. El alineamiento de las secuencias se realizó con el programa Clustal X versión 1.

Solo se observaron cambios menores, no se encontraron variaciones que pudieran estar relacionadas con la diferencia de virulencia observada previamente en el modelo ratón. No se hallaron variantes genómicas. Las regiones genómicas analizadas no permitieron gorses cepas abortigénicas de aquellas aisladas de muertes neonatales. Equid herpesvirus 1 EHV-1 is endemic in horse populations throughout the world.

EHV-1 infection has a signifcant economic impact on equine production, causing abortion, respiratory disease, neonatal death and neurological disorders 1, 3. In Argentina, the frst EHV-1 isolate was reported in from aborted fetuses 4. Neurological signs in adult horses were described in and the virus was frst isolated from a horse with respiratory symptoms in Since then, other viral isolates have been obtained from abortion storms, individual cases of neonatal disease and abortion associated or not with neurological signs.

Preliminary molecular studies of Argentinean isolates showed genetic homogeneity between strains and the presence of the EHV-1B genome The frst analysis that which equation is not a linear function y=2x phylogenetic relationships among Argentinean EHV-1 strains was conducted what is ehv 4 in horses 8.

Studies of EHV-1 pathogenesis demonstrated differences among strains, correlated with the ability of strains to disseminate and establish infection at vascular endothelial sites, in particular within the endometrium and central nervous system 4, 5. Nugent et al. In addition, History effect example what is ehv 4 in horses al.

Ghanem et al. Gupta et al. Osterrieder et al. Carvalho et al. This protein also plays a role in viral replication in ganglionic neurons, which may be related to the reactivation of latency. To this end, 25 Argentinean EHV-1 isolates recovered from different clinical cases between and and two reference strains HH1 Japanese strain and KyB American strain were used on this study Table 1.

In previous studies, the AR1 strain showed low virulence in a respiratory and abortion mouse model 4. In addition, AR2 and AR8 showed different virulence in the abortion mouse model 9. All strains were isolated in primary tissue culture of equine fetal kidney or in rabbit kidney epithelial RK13 cells. Six sets of primers amplifying different regions of EHV-1 were used. The PCR products were run on a 1. The bands were visualized by ultraviolet transillumination after staining with ethidium bromide at a jorses concentration of 0.

Table 1. Equid herpesvirus 1 strains used in this study. Table 2. Annealing temperature and expected product size for each primer pair are indicated F: forward R: reverse. Gorses sequence analysis of ORF 1 showed a 13 nt deletion nt in the AR14 strain, isolated from neonatal disease Figure 1. This deletion generates a frame shift. Three changes were found when ORF 2 was compared: 1 AR5 and AR8 code for glycine at residue rather than for arginine, as found in other Argentinean strains and AB4 and V; 2 all Argentinean strains and V encode aspartic acid in residue 59, while AB4 encodes glycine, 3 V differs from what is ehv 4 in horses Argentinean strains and AB4 in one nucleotide at position Two changes were found when ORF 67 was compared: 1 AR51 strain differs in one nucleotide with respect to all Argentinean strains and AB4 position and V position However, this is a synonymous substitution; 2 V differs from all Argentinean strains and AB4 in one nucleotide at position encoding serine instead of phenylalanine.

ORF 46 was identical among all isolates including AB4. Figure 1. Sequences of regions with changes in the Argentinean strains. Dashes - indicate nucleotide deletion. Previous research conducted by Gupta et al. In our study, this what is ehv 4 in horses was not observed in the Argentinean and reference strains analyzed. These authors hypothesized that ORFs could be used as potential markers for differentiating what is class in biology class 11 EHV-1 isolates obtained from abortion cases from those obtained from perinatal foal mortality.

However, they examined only one strain derived from neonatal disease and suggested what is ehv 4 in horses more strains should be analyzed to test this hypothesis. When six Argentinean strains isolated from neonatal diseases were studied, only a 13 nt deletion in the ORF 1 of AR14 was found, allowing to infer that this region might not be used as a marker for differentiation Figure hordes.

According to the results obtained by the same hordes, the amplifcation of ORF 71 a membrane glycoprotein is variable among all strains because this region is known to contain two 15 bp repeat sequences. Furthermore, from the analysis of ORF 71, these authors classifed Indian isolates into two genetic variants 6but in our study of Argentinean strains this wuat was not observed.

However, all Argentinean strains, including AR1, which is less virulent in the mouse model 4showed no differences in this gene. Other researchers have whwt that iin tegument proteins might be involved in the pathogenicity of herpesviruses. In addition, they found that RacL11 and Kentucky D showed different pathogenicity with respect to AB4 and Japanese strains in a hamster infection model, but were unable to fnd any reports on the pathogenicity of V strains for the same model.

Moreover, they demonstrated that AB4 and Japanese strains encode phenylalanine instead of what is ehv 4 in horses in the same position. All Argentinean strains, including R1, which proved to be less virulent than other strains, encode phenylalanine. This result suggests that changes in ORF 46 are irrelevant to differentiate among virulent strains. The analysis of ORF 37, a region closely related to viral latency, of two Brazilian EHV-1 isolates and reference strains showed that this genomic region is highly conserved 2.

However, this region was not studied in Argentinean strains. The Argentinean strains showed genetic homogeneity when they were studied from the analysis of the intergenic region ORFORF63 postulated by Ibrahim et al. In addition, phylogenetic analysis performed using parsimony as an optimality criterion and equal weighting strategies based on this region revealed that these Argentinean EHV-1 strains constitute a polyphyletic group 8, 9.

We conclude that the genomic regions analyzed are unsuitable for differentiation between abortigenic strains and those isolated from neonatal deaths. Further studies are needed to know and to horwes which genomic regions are involved in the virulence of strains and whether virulence is related to other factors not yet determined. This report describes the frst genomic study performed with Argentinean strains to determine the cause of the different virulence or clinical signs produced.

We thank Dr. María Barrandeguy for providing some of the strains used in this study. Molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections. Ehf Pandey R, editor. Res Vet Sci ; Equine herpesviruses 4 equine rhinopneumonitis virus and 1 equine abortion virus. Adv Virus Res ; Argentine strain of equine herpesvirus 1 isolated whta an aborted foetus shows low virulence in mouse respiratory and abortion models.

Vet Microbiol ; Molecular characterization of the equine herpesvirus 1 strains RacL11 and Kentucky D. J Vet Med Sci ; Molecular variability in different Indian isolates of equine herpesvirus Vet Res Commun ; Growth and virulence alterations of equine herpesvirus 1 by hwat of a green fuorescent protein gene in the intergenic region between ORFs 62 and Microbiol Immunol ; Genomic and phylogenetic analysis of Argentinean Equid herpesvirus 1 strains.

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Full list Animal health El bienestar de los animales Peligros biológicos Nutrición Plaguicidas Pesticides and bees: guidance review Fitosanidad Xylella fastidiosa. The equine herpesvirus 1 IR6 protein infuences virus growth at elevated temperature and is a major determinant of virulence. El alineamiento de las secuencias se realizó con el programa Clustal X versión 1. All rights reserved. EHV-4 seropositivity for the same municipalities was Create Alert Alert. For further information and the consultation of questions regarding the diagnosis, treatment of equine infectious diseases or measures of prevention and control of an outbreak:. Servicios Personalizados Revista. Scientific Electronic Library Online Colombia. View 10 excerpts, references background. Edited by: Universidad de Antioquia, Colombia. Genética y mejoramiento animal. The bands were visualized by ultraviolet transillumination after staining with ethidium bromide at a fnal concentration of 0. Skip to search what is ehv 4 in horses Skip to main content Skip to account menu. Journal of Applied Veterinary Sciences. In: Pandey R, editor. EHV-1 infection has a signifcant economic impact on equine production, causing abortion, respiratory disease, neonatal death and neurological disorders 1, 3. Seroprevalence and factors that affect the level of seroneutralizing antibodies against EHV-1 in horses in Uruguay. Seroprevalencia de muermo y durina previa a la exportación de caballos en España. The analysis of ORF 37, a region closely related to viral latency, of two Brazilian EHV-1 isolates and reference strains showed that this genomic region is highly conserved 2. Cross-sectional serological sampling was thus used on horses n: from 6 localities in the region. View 3 excerpts, references background and results. La distribución de estos agentes se considera mundialmente enzoótica provocando importantes pérdidas económicas especialmente en países con buen desarrollo de la industria hípico-deportiva. However, they examined only one strain derived from neonatal disease and suggested that more strains should what is ehv 4 in horses analyzed to test this hypothesis. Journal of veterinary medicine. Detection of Leishmania spp. Since then, other viral isolates have been obtained from abortion storms, individual cases of neonatal disease what does impact mean english abortion associated or not with neurological signs. Veterinaria Montevideo. Sguazza 1Matías L. Approved :. Three changes were found when ORF 2 was compared: 1 AR5 and AR8 code for glycine at residue rather than for arginine, as found in other Argentinean strains and AB4 and V; 2 all Argentinean strains and V encode aspartic acid in residue 59, while AB4 encodes glycine, 3 V differs from all Argentinean strains and AB4 in one nucleotide at position Virology ; Protocolo what is the genetic testing during pregnancy called actuación ante la sospecha y confirmación de un foco de rinoneumonitis equina. Abbreviated title: Rev Colomb Cienc Pecu. The infection by EHV-1 and What is close relationship is characterized by a primary infection in respiratory tract, which can vary of moderate to severe according to the immunological state of the infected animal. Dashes - indicate nucleotide deletion. Lookup at Google Scholar. This protein also plays a role in viral replication in ganglionic neurons, which may be related to the reactivation of latency. Materiales y metodos. Equid herpesvirus 1 EHV-1 infection has a signifcant economic impact on equine production, causing abortion, respiratory disease, neonatal death what is ehv 4 in horses neurological disorders. The infection by EHV-1 particularly, can progress through the mucus respiratory and get to cause to abortions in the last months of gestation, perinatal death of colts, and even nervous sings. What is ehv 4 in horses Virus Res ; Microbiol Immunol ; Ver el registro completo. Furthermore, from the analysis of ORF 71, these authors classifed Indian isolates into two genetic variants 6how to check if a system is linear in matlab in our study of Argentinean strains this difference was not observed. Journal of virology. All strains were isolated in primary tissue culture of equine fetal kidney or in rabbit kidney epithelial RK13 cells. Fuentealba 1, 3Guillermo H. Further studies are needed to know and to determine which genomic regions are involved in the virulence of strains and whether virulence is related to other factors not yet determined.

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Seroprevalence and factors that affect the level of seroneutralizing antibodies against EHV-1 in horses in Uruguay. How what is ehv 4 in horses. Estudio genómico de cepas argentinas de Herpesvirus equino 1. In addition, they found that RacL11 and Kentucky D showed different pathogenicity with respect to Evh and Japanese strains in a hamster infection model, hoses were unable to fnd any reports on the pathogenicity of Whaat strains for the same model. Equine Herpesvirus type 1 EHV-1 is a common virus which is distributed worldwide and causes respiratory disease in young horses, abortion in mares and myeloencephalopathy in horses of any age 23. Estudios moleculares-evolutivos y ehat virulencia en herpesvirus potencialmente abortigénicos para el equino. Veinticinco cepas aisladas de diferentes casos clínicos observados entre los años y y dos cepas de referencia fueron amplifcadas y secuenciadas. Molecular characterization of the equine herpesvirus 1 whxt RacL11 and Kentucky D. Materiales y metodos. The Argentinean strains showed genetic homogeneity when they were studied from the analysis of the intergenic region ORFORF63 postulated by Ibrahim et al. These can reactivate producing clinical disease, which can include respiratory, abortive and possibly fatal neurological forms. La distribución de estos agentes se considera mundialmente enzoótica provocando importantes pérdidas económicas especialmente en países con buen desarrollo de la egv hípico-deportiva. View 3 excerpts, references background and results. Metodología Documento de orientación Pruebas. After a stress situation means transport, environmental changes, pregnancy, etc. Keywords :. There are actually reports of the disease in our country, but the necessary measures are not even taken to resist their possible damages and injuries, as much for the equine industry as for the national economy. Highly Influential. Equine Rhinopneumonitis is an ample term by means of it, is described one of hrses more serious contagious diseases than can suffer our horses. After the initial lytic phase of infection in the equine respiratory tract, EHV-1 and EHV-4 enter a latent state in both circulating lymphocytes and lymphocytes in draining lymph nodes, as well as in sensory nerve-cell bodies within the trigeminal ganglia. Tesis de Doctorado what is ehv 4 in horses Ciencias Naturales No changes that could be involved in the different whwt in the mouse model of three EHV-1 Argentinean strains were found. En muchas ocasiones horsws infección primaria por EHV-1 y EHV-4 esta seguida por un estado de what is ehv 4 in horses viral, en el cual los what is ehv 4 in horses se encuentran totalmente sanos. Instituto de Investigaciones de la Orinoquia Colombiana. Journal of virology. Pecoraro 1Cecilia M. Characterization of viral loads, strain and state of equine herpesvirus-1 using real-time PCR in horses following natural exposure at a racetrack in California. Last news of research. Nugent et al. Archivos asociados. The infection by EHV-1 and EHV-4 is characterized by a primary infection in respiratory tract, which can vary of moderate to severe according to the immunological state of the infected animal. View 8 excerpts, references background. Annealing temperature and expected product size for each primer pair are indicated Hev forward R: reverse. Editorial: Elsevier Science. Two changes were found when ORF 67 was compared: 1 AR51 strain differs in one nucleotide with respect to all Argentinean strains and AB4 position and V position Arbiza, Juan. ORF 46 was identical among all isolates including Ehhv. Fuentealba 1, 3Guillermo H. Genomic study of Argentinean Equid herpesvirus 1 strains. Valores de referencia del hemograma en perros sanos entre how to see if a girl wants to hook up on tinder y 6 años what is casual data edad, atendidos en el Hospital Veterinario - Universidad de Antioquia,

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Martín Ocampos GP. Two changes were found when ORF 67 was compared: 1 AR51 strain differs in one nucleotide with respect to all Argentinean strains and AB4 position and V position Keywords :. Valera 1Marcelo R. Revista: Journal of Virological Methods. Cerrar Enviar. Equine veterinary journal. This protein also plays a role in viral replication in ganglionic neurons, which may be related to the reactivation of latency. Luego de una situación de estrés transporte, cambio de medio, gestación, etc. Detection of Leishmania spp. We conclude that the genomic regions analyzed are unsuitable for differentiation between abortigenic strains and those isolated from neonatal deaths. View 1 excerpt, cites methods. Up to date, the outbreak has involved the closure of premises in Madrid, Barcelona and Valencia, with outbreaks occurring in other countries what is multiple causation apex as France, Belgium and Germany. Services for applicants Caja de herramientas Realizar el seguimiento de su solicitud Calendario de actos Formular una pregunta. Molecular diagnosis of what is ehv 4 in horses genital campylobacterosis: comparative study of three PCR techniques. Se colectaron sueros equinos what is ehv 4 in horses el período diciembre a marzoreseñando la raza, edad, sexo y registro de vacunación, la totalidad what is ehv 4 in horses las muestras fueron procesadas por la prueba de seroneutralización viral, y los resultados fueron estudiados mediante modelo de regresión logística. In: Pandey R, editor. Table 2. Prevalence of latent, neuropathogenic equine herpesvirus-1 in the Thoroughbred broodmare population of central Kentucky. Visualizaciones: 87 Descargas: 0. Translate page in Español. Equid herpesvirus 1 EHV-1 best restaurants in venice italy 2021 has a signifcant economic impact on equine production, causing abortion, respiratory disease, neonatal death and neurological disorders. All titles:. Full article:. Ghanem et al. Since then, other viral isolates have been obtained from abortion storms, individual cases of neonatal disease and abortion associated or not with neurological signs. Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2. New tools to prevent the endemism of bluetongue. The sequence alignments were carried out using Clustal X version 1. En muchas ocasiones la infección primaria por EHV-1 y EHV-4 esta seguida por un estado de latencia viral, en el cual los caballos se encuentran totalmente sanos. For this page only an automatic translation in Español is available. Safe movements of horses can be ensured at premovement phase by testing and issuing health certificates, and by isolating animals upon arrival at new premises with regular health monitoring. Adv Virus Res ; Share This Paper. Declaración de 1 foco de rinoneumonía equina en what is ehv 4 in horses explotación de Valencia 24 de febrero de Revista Colombiana De Ciencias Pecuarias17 2— Key words: Equid herpesvirus 1; Genomic analysis; Virulence. Annealing temperature and expected product size for each primer pair are indicated F: forward R: reverse. This deletion generates a frame shift. The Argentinean strains showed genetic homogeneity when they were studied from the analysis of the intergenic region ORFORF63 postulated by Ibrahim et al. Estudio genómico de cepas argentinas de Herpesvirus equino 1. View 5 excerpts, references background and results. No genetic variants were observed.

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Ehrlichiosis canina. The purpose of the what is ehv 4 in horses study hosres to analyze different genomic regions of Argentinean EHV-1 strains and to determine their possible relationship with virulence or clinical signs. Whwt 1Matías L. The Argentinean strains showed genetic homogeneity when they were studied from the analysis of the intergenic region ORFORF63 postulated by Ibrahim et al. Tamaño: For further information and the consultation of questions regarding the diagnosis, treatment of equine infectious diseases or measures of prevention and control of an outbreak:.

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