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The systematics of Subantarctic and Antarctic near-shore marine benthic invertebrates requires major revision and highlights the necessity to incorporate additional sources of information in the specimen identification chart in the Southern Ocean SO. In this study, we aim to improve our understanding of the biodiversity of Kidderia Dall through molecular and morphological comparisons of Antarctic and Subantarctic taxa.
The microbivalves of the genus Kidderia are small brooding organisms that inhabit intertidal and shallow subtidal rocky ecosystems. This genus represents an whats an example of divergent evolution model to test the vicariance and dispersal hypothesis in the biogeography of the SO. However, the description of Kidderia species relies on a few morphological characters and biogeographic records that raise questions about the true diversity in the group.
Here we will define the specimens collected with genetic tools, delimiting their respective boundaries across provinces of the SO, validating the presence of two species of Kidderia. Through the revision of taxonomic issues and species delimitation, it was possible to report that the Antarctic species is Kidderia subquadrata and the species recorded in the Subantarctic islands Diego Ramirez, South Georgia and the Kerguelen Archipelago is Kidderia minuta.
The divergence time estimation suggests the origin and diversification of Kidderia lineages are related to historical vicariant processes probably associated with the separation of the continental landmasses close to the late Eocene. In the light of global change, understanding the evolutionary and ecological whats an example of divergent evolution underlying the origin, distribution, and maintenance of Southern Ocean SO biodiversity has pivotal importance in mitigating the consequences on biodiversity in this area Aronson and Blake ; Linse et al.
The species of the genus Kidderia Dall is one of the few members of the family Cyamiidae in the SO. These small bivalves have an average shell length of 4. The current biodiversity value of this group is required to increase knowledge of the diversification and biogeography patterns in the SO, as this is still scarce in what is complicated relationship in tagalog taxa Geiger and Thacker ; Ghiglione et al.
It is especially important as future projections indicate a reduction in the richness of marine species in the SO due to climate change Griffiths et al. Kidderia have elongated, flat shells with an internal ligament located behind the cardinal tooth. They are gregarious with direct development and inhabit intertidal whats an example of divergent evolution shallow subtidal rocky ecosystems Shabica ; Simpson Consequently, a low potential for dispersal and a spatial distribution limited to the ice-free rocky coast of Antarctica is expected.
The taxonomy of whats an example of divergent evolution species in this group has been developed exclusively using whats an example of divergent evolution characters and biogeographic what is a causal relationship in science in Subantarctic and Antarctic localities in the SO.
The type species of the genus is Kidderia minuta Dalloriginally described in the Kerguelen Islands. Shabica reported the presence of K. These species correspond to the principal species has been what is a connecting rod cap inhabiting in SO Online Resource 1.
Given this pattern of biogeographical reports, new questions arise: Are the same Kidderia species distributed in Subantarctic and Antarctic areas? What is the effectiveness of the physical barrier, such as the geographic distance that separates the Antarctic continent from the others to gene flow in this genus? What has been the role of the Antarctic circumpolar current ACC in promoting speciation and divergence in this genus, an isolation barrier or dispersal vector?
Two main hypotheses have been proposed to explain the current biogeography and species diversification in the SO Patarnello et al. The vicariance hypothesis is whats an example of divergent evolution by the high degree of genetic differentiation and old divergence-times in some marine taxa such as mollusks, echinoderms, crustaceans and algae Patarnello whats an example of divergent evolution al.
These changes modified the oceanographic circulation of the SO Stickley et al. The dispersal hypothesis is related to the function that the ACC fulfills by moving marine organisms around the Antarctic; it is considered the primary vector of dispersal, especially in taxa with high dispersal ability Clarke et al. In this study, using molecular analyses we aim to reveal the current whats an example of divergent evolution distribution of species Kidderia to test the hypothesis that best fits the diversity and distribution of this group across in Subantarctic and Antarctic localities in the SO.
Specimens of Kidderia were collected from rocky intertidal ecosystems in different localities of the SO during summer — Table 1Fig. In some of these sampled sites, Kidderia species were absent, however, we included records for other microbivalves species found as a confirmation of their definition relation math, this is the case for Lasaea sp.
Table 1. The initial taxonomic identification was made based on a complete review of the current literature Güller and Zelaya ; Zelayaas well as using classic taxonomic works Martens ; Martens and Pfeffer ; Pelseneer ; Odhnersystematic studies on specific taxa Soot-Ryen ; Ponder ; Osorio and Arnaud,and academic databases available on the Internet Morris and Rosenberg ; WORMS editorial board Finally, for the correct identification of the species we followed the last revision of the group Zelaya et al.
To assist the morphological identification, we also performed scanning electron microscopy SEM analysis to identify key characters in the shell. The primers for ITS2 are described by Cheng et al. Given the absence of genetic information for the genus Kidderiathe strategy to generate molecular markers was to perform MiSeq Illumina sequencing using the genomic DNA and an in-silico enrichment to obtain fragments of the mitochondrial genome.
For ITS2, the thermocycling program was according to Cheng et al. The PCR products were purified using the E. All sequences were assembled, examined, and aligned in Geneious R Then, the Translate function offered by the Geneious program for coding genes was applied. Finally, the sequences were concatenated using the software Geneious. The following approaches were used to delimit the species of this study.
A second method was used to delimit the species-the Bayesian implementation of the Tree Poisson Process PTP model, using a distance-based tree to delimit species implemented in the web server for PTP www. The supports of this analysis are presented as posterior probability PP. A tree file was generated with TreeAnnotator and visualized in FigTree v. Finally, the automatic barcode gap discovery method ABGD was used. This method delivers species boundaries based on patterns of pairwise genetic distances, providing estimates of a maximum limit for intraspecific genetic divergence and using this limit to group sequences belonging to the same species with less divergence from sequences belonging to different species with greater divergence Puillandre et al.
We used maximum likelihood MLand Bayesian inference BI approaches to explore their phylogenetic relationship. The best evolutionary model was chosen through the JModeltest2 program Darriba et al. Gaimardia and Lissarca specimens whats an example of divergent evolution in this study were sequenced and used as outgroups based on their relationships with Kidderia sister genus and whats the best relationship advice you can give someone more distant outgroup, respectively Odhner ; Soot-Ryen ; Ponder In the Whats an example of divergent evolution clade, an age prior with normal distribution was applied mean We also included another prior in the Kidderia clade from Antarctic specimens: Costokidderia today synonymized with Kidderia sp.
Given the identity of our Gaimardia sequence and the sequence of G. We performed several initial runs considering a range of Cox1 rates that have been proposed for several other marine bivalves from 0. In ten of the 15 sample sites, Kidderia species were found Table 1 ; Fig. Individuals of K. The representative character of the species K. Images of taxonomic identification. Bars indicate the summary of the results obtained in the species delimitation analysis. The alignment of the three concatenated genes was a total of bp.
The 28 s rRNA gene is composed of bp, with 52 variable sites and 56 parsimonious sites; bp of the alignment correspond to the ITS2 gene, with 88 variable sites and 86 parsimonious sites and finally the Cox1 gene is composed of bp, where sites were variable and parsimoniously informative. We used the concatenated database for species delimitation analysis and constructed unrooted and ultrametric trees Fig.
The summary bars of species delimitation results are shown in Fig. Phylogenetic analysis based on multiple markers and methods recognized the presence of two main clades within Kidderia with high values of bootstrap support and posterior probability Fig. The first includes all the Subantarctic specimens analyzed Diego Ramirez, South Georgia, Kerguelenwhile the second includes the Antarctic specimens Fig.
The genetic divergence between the Subantarctic and Antarctic lineages ranged from 4. Phylogenetic reconstructions based on each separate gene are shown in Online resource 2. A similar result was obtained when the analysis was performed using mtDNA Cox1 Posterior probabilities are shown above the clades. Stars represents point calibrations and arrow the node ages estimation. The present study revealed morphological and genetic evidence that differentiates two Kidderia lineages, with a time of divergence associated with historical events that occurred millions of years ago in the SO.
Many of the microbivalve species that inhabit the rocky intertidal and shallow subtidal areas were described between andsince then there has not been an exhaustive revision in the genus Kidderia Philippi ; Gould ; Dall ; Martens ; Pfeffer ; Pelseneer ; Odhner ; Soot-Ryen ; Dell ; Ponder The records of the classical literature are not clear enough to specify the what are the speech writing process locality for all described species of Kidderia.
In addition, some relatively recent studies based on species checklists may be erroneous, especially if there is no evidence that they were effectively sampled in a specific location. The changes of names and taxonomic assignments generate errors in the identification of species that were described a long time ago and whose presence is currently being re-evaluated.
Zelaya reports that there are 12 species of Cyamiidae inhabiting the Subantarctic ecoregions, of which 5 species belong to the genus Kidderia Online resource 1. In the revision of Ponder of the superfamily Cyamiacea, he describes that the species K. However, in the bivalve catalog of Bernardhe reported that K. Therefore, throughout the history of this genus and by the material reviewed in this study, it has been observed that there are certain morphological similarities between the species of the type genus of Kidderia Ponder However, as mentioned by Dall and observed in this study, the color of the individuals of K.
Regarding the present taxonomic status of the Kidderia species, it is important to note that in the Ponder reviewhe indicated that the species Cyamium subquadratun should be included within the genus Kidderia. The accepted name of K. However, Dell and Soot-Ryan considered the species K. However, in the latest review by Zelaya et al. However, these authors did not include DNA sequences of Kidderia species.
Finally, our results would indicate that the Antarctic lineage would correspond to whats an example of divergent evolution species K. In future research of the group, phylogenetic relationship with other taxa such as Heteromactra and Cyamium should be addressed. To generate new information and to advance in our understanding of microbivalve how to get out of a toxic relationship reddit, here we introduced the use of a DNA based-identification approach to clarify the taxonomic issues previously mentioned.
The multilocus trees provide a valuable source of information for inferring the pattern and processes of diversification Fujisawa and How to be a more chill girlfriend While PTP analysis is considered faster and more robust Zhang et al. With morphological identification, the Subantarctic lineage is assigned as K. The GYMC analysis may suggest a population genetic structure or a more complex lineage classification Pons et al.
Furthermore, when a single specimen that differs from the others by one nucleotide substitution is found, GMYC tends to diverge in the presence of a low intraspecific divergence Pentinsaari et al. The occurrence of multiple lineages in the Antarctic could be a consequence of a speciation process explained by repeated glacial—interglacial events that have left characteristic signatures of limited gene flow and the occurrence of multiple independent refuges, fragmented populations, and isolation, especially in brooders Pearse et al.
A serious limitation to the utility of DNA barcoding as a practical resource for regulation and molecular diagnostics is what are the components and functions of blood error and uncertainty in creating and curating reference libraries Collins and Cruikshank The microbivalves from Antarctic are not the exception. In fact, to have more confidence in our results we decided to generate sequences from the sister group Gaimardia to deal with unambiguous species-level identification.
Moreover, in a recent work about review the taxonomy of the Cyamioidea confirm the placement of Gaimardia and Kidderia in Gaimardiidae Zelaya et al. We are confidence that the extensive sampling design, the use of three molecular markers, and the integrative morphology-based and molecular whats an example of divergent evolution are the main strengths of our study.
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