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Effect of dietary intake of phenolic compounds from mango peel extract on growth, lipid peroxidation and antioxidant enzyme activities in zebrafish Danio rerio. Cynthia E. Gustavo A. José B. Heredia 3. Four experimental diets were formulated with 50,and mg of phenolic compounds PCs kg -1 feed, derived from mango peel. The control diet did not contain PCs. A total of male zebrafish Danio rerio average weight: mg were fed for eight weeks to assess the ability of PCs to prevent lipid peroxidation and enhance antioxidant status.
Growth performance was calculated at the end of the experimental trial. Lipid peroxidations in muscle and antioxidant enzyme activity in the liver were evaluated at the end of the experiment. There was no significant difference in growth performance among treatments. Malondialdehyde MDA levels in muscle were significantly lower in fish fed diets containing 50 and mg of PCs per kg of feed. Incorporation of PCs into zebrafish diet did not have any significant effects on glutathione peroxidase and superoxide dismutase activity.
However, catalase CAT activity increased significantly in fish with diets containingand mg of PCs kg -1 feed. These results suggest a potential protective effect against in vivo lipid peroxidation and CAT-modulating effects. Keywords: Danio rerio; mango peel; phenolic compounds; antioxidant enzymes; lipid peroxidation; oxidative stress. Lipid peroxidation products, such as lipid peroxyl radicals and malondialdehyde MDA, a good indicator of oxidative damage to lipidscan affect the nutritional quality e.
Assessment of oxidative stress indicators in farmed fish can be used as an index of welfare and muscle quality of fish of commercial interest Sicuro et al. Analyses of oxidative stress indicators in different tissues can be performed to provide additional information about fish health Poli, For example, the fish liver has an important role in detoxification, and its enzymatic antioxidant defense system helps to maintain redox homeostasis Li et al. In the absence of adequate antioxidant enzyme activities, MDA concentration increases in fish muscle, which diminishes its quality.
Mango Mangifera indica contains phenolic compounds PCs with high antioxidant activity Velderrain-Rodríguez et al. Mango cv. There are multiple in vitro and in vivo studies reporting the anti-inflammatory, anti-cancer, anti-diabetic and anti-obesogenic potential of mango peel PCs Shah et al. Therefore, this study aimed to evaluate the ability of mango peel PCs to delay lipid peroxidation in muscle and enhance antioxidant status in the liver of zebrafish Danio reriowhich is considered a good aquaculture model.
Mango peels Mangifera indica cv. Ataulfo were collected from Pure Mango S. Mango peel PCs were extracted according to Sekhon-Loodu et al. Total phenolic content TPC was determined with the Folin-Ciocalteu reagent using gallic acid as standard. The preparation of the experimental diets was carried out as follows: fish what does link mean in peel, fish oil, dextrin, cellulose, soy lecithin, alginate, mineral premix, vitamin premix, vitamin C and synthetic antioxidant butylated hydroxytoluene BHTwere used to prepare the control diet.
Four additional diets were prepared and supplemented with PCs from the mango peel extract MPE at concentrations of 50,and mg PCs kg -1 feed. The macronutrients fish meal, dextrin, cellulose and alginate were mixed in a model AT Hobart mixer Offenburg, Germanyand the micronutrients mineral premix, vitamin premix, vitamin C and BHT were added.
Fish oil and soy lecithin were added. Finally, PCs that had been homogenized in warm water were added until a homogeneous mixture was obtained. Table 1 Ingredients, proximate composition and PCs content of the experimental diets administered to zebrafish Danio rerio. Ingredients and diets were analyzed to determine moisture, crude protein, crude fat and ash content method Moisture was determined using a Craft stove method The PCs content in the experimental diets was analyzed to determine the final concentration in zebrafish Danio rerio feed.
Briefly, diets 0. PCs content was determined with the Folin-Ciocalteu reagent using gallic acid as standard. A completely randomized experimental design with three replicates per treatment was used. Zebrafish were placed in 15 aquarium tanks 6 L in groups of eight fish; each tank had a static renewal system. The effect of PCs on growth performance and the antioxidant response was evaluated using the following feeding protocol: zebrafish were fed twice a day manually and h to apparent satiety for eight weeks.
Fish were weighed individually under anesthesia [0. The head, tail and viscera were removed from each fish to quantify lipid peroxidation. On the last day of the experiment, what does link mean in peel were euthanized what does link mean in peel anesthesia with 0. The supernatant was used to determine total protein content and antioxidant enzyme activities.
Total what does link mean in peel content was determined using Bradford's reagent Bradford, and bovine serum albumin as a standard. All enzyme activity results were expressed as specific enzyme activity in U mg -1 protein. Data were evaluated for normality Kolmogorov-Smirnov test and homogeneity of variance Levene's test before statistical analyses. Statistical analyses were run using Statistica v. Eight PCs were identified, five of which were phenolic acids, two flavonoids and a polyphenol, xanthone Table 2.
What is a common law partner entitled to in canada 2 PCs detected in mango peel cv. Table 3 Growth parameters and feed efficiency of zebrafish Danio rerio fed experimental diets for eight weeks. Lipid peroxidation is presented in Fig. Mango peel extract is a rich source of PCs such as phenolic acids and flavonoids, which exhibit high antioxidant fixing a broken relationship both in vivo and in vitro.
The PCs identified in what does link mean in peel study have also been reported by Velderrain-Rodríguez et al. Ataulfo extract. Additionally, Pacheco-Ordaz et al. Other studies have reported the presence of methyl gallate, ethyl gallate, quercetin and isoquercetin in mango peel Dorta et al. In aquaculture, the inclusion of some feed additives such as PCs from plant extracts could negatively affect growth performance and feed efficiency of fish Buyukcapar et al.
In the present study, the predominating compounds of the MPE PCs, which were incorporated into zebrafish Danio rerio feed, were phenolic acids gallic, 2-hydroxycinnamic and protocatechuic acids; gallic acid derivatives such as methyl and ethyl gallateflavonoids quercetin and isoquercetin and a polyphenol xanthone known as mangiferin, which did not show effects what are the various constituents of air class 6 growth performance and feed efficiency.
Our results on growth performance and feed efficiency of zebrafish are in agreement with other studies conducted on gilthead sea bream Sparus aurata and rainbow trout Oncorhynchus mykisswhere PCs extracted from olive oil hydroxytyrosol and hydroxycinnamic acids and purple maize anthocyanins were supplemented in the diets of these species Sicuro et al. Condensed tannins are considered as antinutrients for aquatic organisms since they can form stable complexes with proteins and can lead to reduced protein digestibility if they bind with digestive proteases, which is directly related to a reduction in growth performance and feed efficiency in farmed fish Omnes et al.
Therefore, the MPE-derived PCs used here are safe to administer to zebrafish in concentrations of up to mg per kg of feed. Oxidative stress can promote the peroxidation of PUFAs in fish flesh. Lipid peroxidation is an important process that can lead to decreased quality of muscle, decreased shelf life, loss of nutritionally relevant compounds e. The major PCs found in MPE, such as mangiferin, quercetin, gallic acid, and its derivatives, are characterized by high antioxidant capacity, which can what is standard deviation class 11 economics oxidative stress through various mechanisms.
There is evidence confirming that mangiferin, quercetin, gallic acids, and its derivatives have the iron-chelating ability and can scavenge ROS which constitutes their main antioxidant mechanisms preventing lipid peroxidation in vitro Boadi et al. Other in vitro studies report that the addition of grape PCs catechin and procyanidins and quince extracts containing procyanidin B dimer and hydrocycinnamic acids prevent lipid peroxidation in the muscle of different fish species during storage Pazos et al.
There are also studies that have demonstrated the effectiveness of PCs from plant extracts such as feed additives on preventing lipid peroxidation in animal tissue Surai, However, there are only a limited number of studies on the use of PCs from plant extracts as a feed additive with antioxidant properties on the prevention of lipid peroxidation in the muscle of farmed fish.
For example, Villasante et al. Another study indicated that dietary administration of an olive oil extract rich in tyrosol and hydroxytyrosol decreased MDA levels in muscle of gilthead sea bream Sicuro et al. The majority of the available reports on PCs in aquaculture have mainly focused on their use as immunostimulants Nootash et al. Our data suggest that MPE PCs significantly mitigate lipid peroxidation in zebrafish, which merits further investigation in fish that are used for human consumption to validate their use as feed supplements conclusively.
During oxidative stress, tissues respond by inducing enzymatic and non-enzymatic antioxidant defense mechanisms. Sellamuthu what are the causes and effects of air pollution in china al. Nrf2 is a transcription factor involved what does link mean in peel the cellular antioxidant response, modulating gene expression of various enzymes casula central medical centre proteins.
H 2 O 2 is the main cellular precursor of the hydroxyl radical which is considered as the most biologically active free radical, hence why the removal of H 2 O 2 is a good strategy against oxidative stress Karadag et al. Supplementation of 50 and mg PCs per kg of feed decreased lipid peroxidation end-products MDA in zebrafish muscle and could, therefore, be used to avoid what does link mean in peel decrease in EPA and DHA in fish flesh destined for human consumption, potentially providing health benefits to consumers.
Based on these findings, MPE PCs could be used as feed additives to preserve the nutritional quality of fish of commercial interest. Further studies are necessary to support these findings. Ardiansyah, A. Natural antioxidants dietary and lipid oxidation analysis in zebrafish Brachydanio rerio tissue. Official methods of analysis.
Association of Official Analytical Chemists, Virginia. Ayala, A. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxynonenal. Oxidative Medicine and Cellular Longevity, Badhani, B. Gallic acid: a versatile antioxidant with promising therapeutic and industrial applications. RSC Advances, 5 35 : Boadi, W. Effect of quercetin and genistein on copper-and iron-induced lipid peroxidation in methyl linolenate.
Journal of Applied Toxicology, 23 5 : Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72 :